細(xì)胞名稱(chēng)：4T1 小鼠乳腺癌細(xì)胞
介紹：4T1 是從410.4瘤株中未經(jīng)誘變篩得的6-硫鳥(niǎo)嘌噙抗性細(xì)胞株。 當(dāng)注射到BALB/c 小鼠中時(shí)，4T1自發(fā)產(chǎn)生高轉(zhuǎn)移腫瘤，可轉(zhuǎn)移到肺，肝，淋巴結(jié)和大腦，同時(shí)在注射部位形成始發(fā)灶。 誘導(dǎo)轉(zhuǎn)移時(shí)不需要摘除始發(fā)灶。 4T1細(xì)胞在BALB/c小鼠中的生長(zhǎng)與轉(zhuǎn)移特性與人體中的乳腺癌十分相近。這種腫瘤是人VI期乳腺癌的動(dòng)物模型。 4T1-誘導(dǎo)的腫瘤在手術(shù)后及未手術(shù)情況下轉(zhuǎn)移的動(dòng)力學(xué)相近，可以用作手術(shù)后及未手術(shù)模型。 跟其他腫瘤模型相比，由于4T1的抗6-硫鳥(niǎo)嘌噙特性，微小的轉(zhuǎn)移細(xì)胞團(tuán)(少到僅僅1個(gè))也可以在許多遠(yuǎn)端器官中檢測(cè)到。 沒(méi)必要數(shù)淋巴結(jié)或稱(chēng)重器官
形態(tài)特性       上皮細(xì)胞
生長(zhǎng)特性      貼壁生長(zhǎng)
傳代方法       消化3-5分鐘。1:2。3天內(nèi)可長(zhǎng)滿。
支原體檢測(cè)      陰性
下面是ATCC的介紹

4T1 (ATCC® CRL-2539™)
Organism    Mus musculus, mouse
Tissue     mammary gland
Product Format     frozen4T1 小鼠乳腺癌細(xì)胞
Morphology     epithelial
Culture Properties     adherent
Biosafety Level     1
Disease     This tumor is an animal stage IV human breast cancer.
Strain     BALB/cfC3H
Applications    
The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer.

4T1-induced tumors can be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics.
Storage Conditions     liquid nitrogen vapor temperature
Derivation    
4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment.
Tumorigenic     Yes
Effects    4T1 小鼠乳腺癌細(xì)胞
Yes, forms tumors and metastasizes in BALB/c mice
Comments    
When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.
The primary tumor does not have to be removed to induce metastatic growth.
Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy that most tumor models. There is no need to count nodules or weight target organs.

Complete Growth Medium     The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (Ref). The Phenol Red-free version of RPMI 1640, is ATCC catalog No. 30-2602.

Subculturing    
NOTE: the cells should not be allowed to become confluent, subculture at 80% of confluence. Remove medium, and rinse with 0.25% trypsin-0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation    
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions    
Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%